IMPROVED MEASUREMENT OF C-13, P-31 J-COUPLING-CONSTANTS IN ISOTOPICALLY LABELED RNA

Authors
LEGAULT P JUCKER FM PARDI A
Citation
P. Legault et al., IMPROVED MEASUREMENT OF C-13, P-31 J-COUPLING-CONSTANTS IN ISOTOPICALLY LABELED RNA, FEBS letters, 362(2), 1995, pp. 156-160
Citations number
29
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
362
Issue
2
Year of publication
1995
Pages
156 - 160
Database
ISI
SICI code
0014-5793(1995)362:2<156:IMOCPJ>2.0.ZU;2-W
Abstract
(3)J(CP) coupling constants have been measured in a 99% C-13,N-15 labe led lead-dependent ribozyme, known as the leadzyme, These coupling con stants were determined by analysis of the intensity of individual cros speaks in a spin-echo difference constant time HSQC experiment, This p rocedure permits improved measurement of the (3)J(C2'P) and (3)J(C4'P) coupling constants in isotopically labeled RNA and yielded valuable i nformation on the beta and epsilon backbone torsion angles in the lead zyme.