PRIMARY STRUCTURE OF PROTEINS FROM THE WING CUTICLE OF THE MIGRATORY LOCUST, LOCUSTA-MIGRATORIA

Wing cuticle from pharate adult locusts, Locusta migratoria, contains several prominent proteins which occur as minor components or are comp letely absent in other cuticular regions. Six of the wing-specific pro teins have been purified and their amino acid sequences determined by combined use of mass spectrometry and automated Edman degradation. Dur ing the sequence determination very long sequence runs (90-121 residue s) were necessary in order to establish the primary structure, All the wing-specific cuticular proteins from locusts contain the repeated sh ort sequence motif -Ala-Ala-Pro-Ala/Val-, which is common for all hith erto sequenced cuticular proteins from pharate locusts. Several of the wing-specific proteins also possess an N-terminal region rich in glyc ine, tyrosine and leucine, characteristic for many locust cuticular pr oteins. Two of the analysed proteins have a conserved 61-residue seque nce in common with a previously sequenced protein from locust wing cut icle and with two proteins from the pharate cuticle of adult Tenebrio molitor. Possible roles for the various sequence motifs are discussed.