This paper reviews a new technique to develop high-resolution three-di
mensional (3-D) images of the larynx using histological sections, Thre
e-dimensional computer-reconstructed histological sections of the cat
are used in this study to evaluate the recurrent laryngeal nerve (RLN)
in its true anatomic course, with emphasis on its relationship to sur
rounding structures (laryngeal framework), A cat model was used becaus
e of specimen availability and technical ease of tissue preparation. C
omputer-reconstructed serial histologic sections add a new dimension t
o the study of laryngeal anatomy, laryngeal trauma, and growth pattern
s of laryngeal cancer, The technical aspects of three-dimensional reco
nstruction and future applications are discussed.