IDENTIFICATION OF GENITAL HUMAN PAPILLOMAVIRUSES IN CERVICAL BIOPSY SPECIMENS - SEGREGATION OF SPECIFIC VIRUS TYPES IN SPECIFIC CLINICOPATHOLOGICAL LESIONS

We have established a critical identification method for the full spec trum of genital human papillomaviruses (HPVs) in clinical specimens. I t was based on the recognition of PstI, BanI and MspI cleavage pattern s of HPV DNA detected by blot hybridization with HPV 58 DNA probe at T m-40 degrees C. By this method, we identified 24 different types of ge nital HPV including 5 novel types (HPV 59, 61, 62, 64 and 67) in the s pecimens collected at one hospital and found almost all the HPVs with the authentic cleavage patterns of their respective prototypes. In 235 cervical biopsy specimens, HPV 6 or 11 was found in exophytic condylo ma acuminatum (15/15) but not in any cervical intraepithelial neoplasi a (CIN) specimens. In contrast, HPV 18, 30, 43, 54, 56, 59, 62, 66 and 67 were identified in CIN 1 (28/71) or 11 (4/56) but not in CIN III, while HPV 16, 31, 33, 35, 39, 51, 52 and 58 were identified in CIN III (83/93) as well as in CIN I (34/71) and 11 (47/56). The result indica tes that heterogeneous genital HPVs prevail all over the world. In add ition, HPV 6 and II are etiologic agents only of exophytic condyloma, whereas the other HPVs are etiologic agents of CIN with the segregatio n of specific HPVs in CIN III. We propose a new clinicopathologic grou ping of genital HPVs founded on nucleotide homology of the HPV genome. (C) 1995 Wiley-Liss, Inc.