ACCEPTOR SPECIFICITY OF DIFFERENT LENGTH CONSTRUCTS OF HUMAN RECOMBINANT ALPHA-1,3 4-FUCOSYL-TRANSFERASES - REPLACEMENT OF THE STEM REGION AND THE TRANSMEMBRANE DOMAIN OF FUCOSYL-TRANSFERASE-V BY PROTEIN-A RESULTS IN AN ENZYME WITH GDP-FUCOSE HYDROLYZING ACTIVITY/
T. Devries et al., ACCEPTOR SPECIFICITY OF DIFFERENT LENGTH CONSTRUCTS OF HUMAN RECOMBINANT ALPHA-1,3 4-FUCOSYL-TRANSFERASES - REPLACEMENT OF THE STEM REGION AND THE TRANSMEMBRANE DOMAIN OF FUCOSYL-TRANSFERASE-V BY PROTEIN-A RESULTS IN AN ENZYME WITH GDP-FUCOSE HYDROLYZING ACTIVITY/, The Journal of biological chemistry, 270(15), 1995, pp. 8712-8722
The acceptor specificity of recombinant full-length, membrane-bound fu
cosyltransferases, expressed in COS-7 cells, and soluble, protein-A ch
imeric forms of alpha 1,3-fucosyltransferase (Fuc-T) III, Fuc-TIV, and
Fuc-TV was analyzed toward a broad panel of oligosaccharide, glycolip
id, and glycoprotein substrates. Our results on the full-length enzyme
s confirm and extend previous studies. However, chimeric Fuc-Ts showed
increased activity toward glycoproteins, whereas chimeric Fuc-TIII an
d Fuc-TV had a decreased activity with glycosphingolipids, compared to
the full-length enzymes. Unexpectedly, chimeric Fuc-TV exhibited a GD
P-fucose hydrolyzing activity. In substrates with multiple acceptor si
tes, the preferred site of fucosylation was identified. Fuc-TIII and F
uc-TV catalyzed fucose transfer exclusively to OH-3 of glucose in lact
o-N-neotetraose and lacto-N-tetraose, respectively, as was demonstrate
d by H-1 MMR spectroscopy. Thin layer chromatography immunostaining re
vealed that FucT-IV preferred the distal GlcNAc residue in nLc(6)Cer,
whereas Fuc-TV preferred the proximal GlcNAc residue. Incubation of Fu
c-TIV or Fuc-TV with VI(3)NeuAcnLc(6)Cer resulted in products with the
sialyl-Lewis(X) epitope as well as the VIM-2 structure. To identify p
olar groups on accepters that function in enzyme binding, deoxygenated
substrate analogs were tested as accepters. All three Fuc-Ts had an a
bsolute requirement for a hydroxyl at C-6 of galactose in addition to
the accepting hydroxyl at C-3 or C-4 of GlcNAc.