C. Duport et al., MOLECULAR CHARACTERIZATION OF PYOCIN S3, A NOVEL S-TYPE PYOCIN FROM PSEUDOMONAS-AERUGINOSA, The Journal of biological chemistry, 270(15), 1995, pp. 8920-8927
The genetic determinant for the soluble pyocin S3 was isolated from a
genomic library constructed in the plasmid pGV1122, of Pseudomonas aer
uginosa strain P12 isolated from a cystic fibrosis patient. The nucleo
tide sequence of a 3270-base pair DNA fragment was determined, and the
two structural genes, pyoS3A and pyoS3I, and the 3'- and 5'-flanking
regions were localized. Transcription (Northern blot) analysis showed
that the two genes were co-transcribed. The genes pyoS3A and pyoS3I co
de for polypeptides of 767 and 154 amino acids, respectively, with cal
culated molecular weights of 81,385 and 17,047. Pyocin S3 was produced
in Escherichia coli from a plasmid and purified as a complex of two c
omponents (S3A and S3I) corresponding to the pyoS3A and pyoS3I gene pr
oducts, respectively. The S3A component, like pyocin S3, had a killing
effect involving DNase activity and was inhibited by the S3I protein.
Comparisons of the predicted amino acid sequence of the two component
s of pyocin S3 to those of pyocins S1, S2, and AP41 indicate that pyoc
in S3 is a new type of S-type pyocin.