APPLICATION OF A DUAL-COLOR DETECTION SCHEME IN THE SCREENING OF A RANDOM COMBINATORIAL PEPTIDE LIBRARY

Selectide Technology is a random synthetic combinatorial library metho d in which millions of random compounds are screened in parallel for t heir ability to bind to a tagged macromolecular target. The library co nsists of millions of beads and each individual bead expresses a uniqu e chemical compound such as a peptide. In the standard enzyme-linked c olorimetric detection scheme, the positive bead which turns color is i solated for microsequencing. In this paper, a dual color detection sch eme using two sequential orthogonal probes is described. This dual col or system enables one to rapidly differentiate false positive beads fr om true positive beads, resulting in a much more efficient use of the microsequencer.