ISOLATION OF GENES WHICH BLOCK NEURONAL DIFFERENTIATION OF TERATOCARCINOMA PCC7 CELLS

Since several viral oncoproteins block differentiation and induce prol iferation of differentiated cells, we developed an expression screenin g method to isolate cDNAs which block neuronal differentiation and ind uce proliferation of teratocarcinoma cells, Mouse E2F1, RNP-1, and RNP -2 (Regulator of Neuronal Proliferation) were isolated using the devel oped screening method, Overexpression of E2F1, RNP-1, and RNP-2 cDNAs in neuronally differentiated teratocarcinoma PCC7 cells results in blo cking differentiation and initiation of proliferation, Also, expressio n of RNP-1 and RNP-2 blocks the expression of neurofilament-L and GAP- 43 genes in PCC7 cells. (C) 1995 Academic Press, Inc.