PROANAL VERSION-2 - MULTIFUNCTIONAL PROGRAM FOR ANALYSIS OF MULTIPLE PROTEIN-SEQUENCE ALIGNMENTS AND FOR STUDYING THE STRUCTURE-ACTIVITY-RELATIONSHIPS IN PROTEIN FAMILIES

A new version of the program PROANAL is described. A multiple linear r egression analysis of the protein structure activity relationship allo ws one to investigate the combinations of protein sites and factors in fluencing the activity. The program also provides the possibility to s eek out protein sites, conservative or variable in variations of physi cochemical characteristics, and regions with high or low values of the se characteristics. PROANAL2 may be useful in the simulation of protei n-engineering experiments and in the search of a number of protein reg ions such as functional sites, secondary structures, solvent-exposed r egions, T- and B-cell antigenic determinants, etc.