SOMATOGENIC AND LACTOGENIC ACTIVITY OF THE RECOMBINANT 22-KDA ISOFORMOF HUMAN PLACENTAL GROWTH-HORMONE

Authors
IGOUT A FRANKENNE F LHERMITEBALERIAUX M MARTIN A HENNEN G
Citation
A. Igout et al., SOMATOGENIC AND LACTOGENIC ACTIVITY OF THE RECOMBINANT 22-KDA ISOFORMOF HUMAN PLACENTAL GROWTH-HORMONE, Growth regulation, 5(1), 1995, pp. 60-65
Citations number
29
Categorie Soggetti
Endocrynology & Metabolism
Journal title
Growth regulation → ACNP
ISSN journal
0956523X
Volume
5
Issue
1
Year of publication
1995
Pages
60 - 65
Database
ISI
SICI code
0956-523X(1995)5:1<60:SALAOT>2.0.ZU;2-E
Abstract
During pregnancy, the human placenta secretes a variant of pituitary g rowth hormone (hGH-V) differing in sequence from the pituitary growth hormone (hGH-N) by 13 amino acids substitutions, HGH-V replaces hGH-N in the maternal bloodstream during the second half of pregnancy. HGH-V is produced in two, possibly three, isoforms: a 22 kDa form, a glycos ylated 25 kDa form, and a probable 26 kDa form resulting from alternat ive splicing of the mRNA. Here we have studied the somatogenic and lac togenic activity of recombinant 22 kDa hGH-V isoform produced in Esche richia coli and compared it with the 22 kDa form of hGH-N. The two var iants exert a similar somatogenic effect on rabbit and rat cells, but differ as regards their lactogenic activity in the rat.