EFFICIENT ANALYSIS OF CYTOCHROME P4501A CATALYTIC ACTIVITY, PORPHYRINS, AND TOTAL PROTEINS IN CHICKEN-EMBRYO HEPATOCYTE CULTURES WITH A FLUORESCENCE PLATE READER

Authors
KENNEDY SW JONES SP BASTIEN LJ
Citation
Sw. Kennedy et al., EFFICIENT ANALYSIS OF CYTOCHROME P4501A CATALYTIC ACTIVITY, PORPHYRINS, AND TOTAL PROTEINS IN CHICKEN-EMBRYO HEPATOCYTE CULTURES WITH A FLUORESCENCE PLATE READER, Analytical biochemistry, 226(2), 1995, pp. 362-370
Citations number
12
Categorie Soggetti
Biology
Journal title
Analytical biochemistry → ACNP
ISSN journal
00032697
Volume
226
Issue
2
Year of publication
1995
Pages
362 - 370
Database
ISI
SICI code
0003-2697(1995)226:2<362:EAOCPC>2.0.ZU;2-P
Abstract
An efficient method for measuring polychlorinated biphenyl-mediated in duction of cytochrome P4501A, using the ethoxyresorufin-O-deethylase ( EROD) assay, and total porphyrins in chicken embryo hepatocyte culture s is described. Hepatocytes were cultured in 48-well plates, assays we re carried out within the wells, and concentrations of the product of the EROD reaction (resorufin), porphyrins, and total proteins were mea sured in the same wells with a fluorescence plate reader. The method d iffers from previous methods developed in this laboratory in that thre e fluorophors were measured in the same well rather than two. (C) 1995 Academic Press, Inc.