Y. Mimaki et al., STEROIDAL GLYCOSIDES FROM THE UNDERGROUND PARTS OF HOSTA-PLANTAGINEA VAR JAPONICA AND THEIR CYTOSTATIC ACTIVITY ON LEUKEMIA HL-60 CELLS, Phytochemistry, 44(2), 1997, pp. 305-310
A new C-22-steroid glycoside was isolated from the underground parts o
f Hosta plantaginea var. japonica, together with a known furostanol sa
ponin and three known spirostanol saponins. The structure of the new s
teroid glycoside was characterized by spectroscopic analysis and acid-
catalysed hydrolysis as 2 alpha,3 beta,16 beta-trihydroxy-5 alpha-preg
n-20(21)-ene-carboxylic acid gamma-lactone -glucopyranosyl-(1-->2)-O-b
eta-D-glucopyranosyl-(1 -->4)-beta-D-galactopyranoside}. The isolated
compounds were assayed for their cytostatic activity on leukaemia HL-6
0 cells. The spirostanol saponins showed cytostatic activity in a dose
-dependent manner with the IC50 values ranging between 1 and 3 mu g ml
(-1). Copyright (C) 1996 Elsevier Science Ltd