S. Micinski et al., AN IMPROVED PLANT WASHING PROCEDURE FOR MONITORING EARLY-SEASON INSECT PESTS IN COTTON, The Southwestern entomologist, 20(1), 1995, pp. 17-24
Two plant washing techniques were compared for efficiency of extractio
n of thrips (primarily Frankliniella fusca (Hinds)) and cotton aphids,
Aphis gossypii Glover, from seedling cotton. The techniques included
a plant washing method previously reported and a modification of this
procedure that included centrifugal flotation. Both techniques were eq
ually effective in extraction of insects from plants grown in a clay l
oam soil, and the techniques were comparable at both high and low inse
ct population levels. A significant increase in sample quality, as mea
sured by time required for quantification of insects after extraction,
was found when centrifugal flotation was included in the procedure. T
ime required for individual sample quantification was decreased by 30
to 69% with the use of centrifugal flotation. For plants grown in a cl
ay soil, there was no advantage in insect recovery efficiency and time
required for sample quantification was significantly reduced on only
one of three sampling dates.