S. Backlund et M. Rantala, LECITHIN PROPANOL-BASED MICROEMULSIONS USED AS MEDIA FOR A CHOLESTEROL OXIDASE-CATALYZED REACTION/, Colloid and polymer science, 273(3), 1995, pp. 293-297
Reverse micelles, Winsor III and IV systems were examined as reaction
media for the enzymatic conversion of cholesterol to cholestenone by c
holesterol oxidase at 298.2 K. The micelles and the microemulsions, st
abilized by soybean lecithin and ethanol or 1-propanol as cosolvent, w
ere characterized with respect to phase behavior and distribution of 1
-propanol between the phases of the Winsor III systems. The used oils
were dodecane, tetradecane, and hexadecane. The Winsor IV systems and
the surfactant-rich phase in the Winsor III systems exhibit bicontinuo
us structures. The reaction yield for the enzymatic conversion perform
ed in a Winsor IV system was much higher than in a Winsor III system o
r in reverse micelles.