CO-APPLICATION of lpha-amino-3-hydro-5-methyl-4-isoxazole-propionate (
AMPA) and 8-bromo cGMP (8-Br-cGMP) which cause long-term desensitizati
on also induces c-Fos and Jun-B expression in Purkinje cells of cerebe
llar slices [Nakazawa K, Karachot L, Nakabeppu Y et al. NeuroReport 4,
1275-1278 (1993)]. Here, we report an increased local induction of Ju
n-B immunoreactivity in Purkinje cells in vivo when electrical stimula
tion of the inferior olive nucleus (IOn) was conjunctively applied wit
h AMPA on the vermis. The present data further supports the idea that
conjunctive heterosynaptic inputs to cerebellar Purkinje cells can tri
gger active gene transcription thus possibly contributing to cerebella
r long-term plasticity. They also demonstrate that Jun-B may be a usef
ul transcriptional marker to study cerebellar coincidence phenomena.