A FED-BATCH CULTURE MODEL FOR IMPROVED PRODUCTION OF GIBBERELLIC-ACIDFROM A WASTE MEDIUM

The kinetics of the production of gibberellic acid (GA3) in convention al batch culture was compared with that obtained in fed-batch cultures at three different feeding regimes with media containing protein N, o ne of which was calculated to maintain a constant biomass, and two oth ers able to promote linear growth. It was found that the production of GA3 improves substantially in the latter two cases, and that the gene ral model applicable to the formation of a secondary metabolite correc tly predicts the results if one dispenses with the assumption of avoid ing the increase in the biomass during the production phase.