Hp. Chen et al., REGIOSELECTIVITY ENHANCEMENT BY PARTIAL-PURIFICATION OF LIPASE FROM ASPERGILLUS-NIGER, Biotechnology letters, 17(3), 1995, pp. 305-308
Lipase from Aspergillus niger was partially purified by a single step
of ion-exchange chromatography and two major fractions I and II could
be obtained. Two substrates, peracetylated 1-methyl and 1-thioethyl be
ta-D-glucopyranosides, were deacetylated by fraction II to give 3-OH d
erivatives as sole products with very high yield, respectively.