REGIOSELECTIVITY ENHANCEMENT BY PARTIAL-PURIFICATION OF LIPASE FROM ASPERGILLUS-NIGER

Citation
Hp. Chen et al., REGIOSELECTIVITY ENHANCEMENT BY PARTIAL-PURIFICATION OF LIPASE FROM ASPERGILLUS-NIGER, Biotechnology letters, 17(3), 1995, pp. 305-308
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
ISSN journal
01415492
Volume
17
Issue
3
Year of publication
1995
Pages
305 - 308
Database
ISI
SICI code
0141-5492(1995)17:3<305:REBPOL>2.0.ZU;2-P
Abstract
Lipase from Aspergillus niger was partially purified by a single step of ion-exchange chromatography and two major fractions I and II could be obtained. Two substrates, peracetylated 1-methyl and 1-thioethyl be ta-D-glucopyranosides, were deacetylated by fraction II to give 3-OH d erivatives as sole products with very high yield, respectively.