Ss. Elsonbaty et al., EXOGENOUSLY EXPRESSED GRANULOCYTE-COLONY-STIMULATING FACTOR (G-CSF) RECEPTOR ON K562 CELLS CAN TRANSDUCE G-CSF TRIGGERED GROWTH AND DIFFERENTIATION SIGNALS, International journal of hematology, 61(2), 1995, pp. 61-68
Human granulocyte colony-stimulating factor (G-CSF) receptor cDNA was
introduced into the erythroleukemic cell line K562, which normally doe
s not express the receptor, using lipofection transfection of a G-CSF
receptor expression plasmid vector, Transfected cells expressed the re
ceptor with a dissociation constant of 130 pmol/L, and a maximum of 11
800 binding sites per cell. Culture of G-CSF receptor-expressing cell
s (GR-K562) in the presence of G-CSF enhanced DNA synthesis and a stim
ulation index of 4.61 was obtained in a H-3-thymidine uptake assay, Fu
rthermore, flow cytometric studies revealed induced expression of CD11
b (29% from 7%), and enhanced expression of CD13 (57% from 27%) on GR-
K562 cultured in the presence of G-CSF, Our findings indicate that the
exogenously expressed G-CSF receptor can deliver signals and function
efficiently on these immature cells on which the receptor is not norm
ally expressed, which suggests the presence of intact intracellular si
gnal transduction pathways that can be used by the ectopically express
ed receptor.