COMPARISON OF 3 CHROMATOGRAPHIC SYSTEMS FOR DETERMINATION OF ORGANIC-ACIDS IN WINE

Three chromatographic systems: ion exchange chromatography, ion exclus ion chromatography and reversed phase chromatography, have been used f or the simultaneous determination of organic acids in wine. The common organic acids were separated using all three chromatographic systems. When an ion exchange column (TSKgel IC Anion-PW) was used, organic ac ids and inorganic anions (Cl- and SO42-) in wine were determined simul taneously without interference. The sharp peaks were obtained when an ion exclusion column (TSKgel OApak-A) was employed. A rapid separation of organic acids has been achieved, within about 7 min, when a revers ed phase column (Zorbax ODS) was used. In ion exclusion and reversed p hase systems combined with UV detection, however, other organic compou nds which have ultraviolet absorption at 210 nm interfered with the de termination of organic acids when practical wine samples were analyzed . IC is an obvious alternative for determination of organic acids in w ine.