LARGE-SCALE PROTOPLAST ISOLATION AND REGENERATION OF ARABIDOPSIS-THALIANA

Authors
Citation
Ar. Wenck et L. Marton, LARGE-SCALE PROTOPLAST ISOLATION AND REGENERATION OF ARABIDOPSIS-THALIANA, BioTechniques, 18(4), 1995, pp. 640-643
Citations number
12
Categorie Soggetti
Biochemical Research Methods
Journal title
ISSN journal
07366205
Volume
18
Issue
4
Year of publication
1995
Pages
640 - 643
Database
ISI
SICI code
0736-6205(1995)18:4<640:LPIARO>2.0.ZU;2-3
Abstract
A simple method for large-scale isolation and regeneration of Arabidop sis thaliana green protoplasts utilizing a novel starting material is presented. This technique takes advantage of shooty/embryogenic masses regenerating from fresh or cultured roots. There is no time-consuming mechanical isolation of starting material. Economical Bacto agar is u sed as an embedding agent. Plating efficiencies are between 5% and 10% . Seventy-six percent of protoplast-derived colonies produce shoots.