ALTERNATIVE TO POLYACRYLAMIDE GELS IMPROVES THE ELECTROPHORETIC MOBILITY SHIFT ASSAY

Authors
VANEK PG FABIAN SJ FISHER CL CHIRIKJIAN JG COLLIER GB
Citation
Pg. Vanek et al., ALTERNATIVE TO POLYACRYLAMIDE GELS IMPROVES THE ELECTROPHORETIC MOBILITY SHIFT ASSAY, BioTechniques, 18(4), 1995, pp. 704-706
Citations number
3
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
18
Issue
4
Year of publication
1995
Pages
704 - 706
Database
ISI
SICI code
0736-6205(1995)18:4<704:ATPGIT>2.0.ZU;2-F
Abstract
In this paper we outline a simplified protocol for the electrophoretic mobility shift assay utilizing TreviGelTM 500, a nontoxic alternative to polyacrylamide. The TreviGel 500 matrix combines the strength and resolution of polyacrylamide with the simplicity and flexibility of ag arose in the casting of gels. Therefore, this method provides a simple , rapid and nontoxic alternative to current protocols for the investig ation of protein:DNA interactions.