TOWARDS LARGE-SCALE METHODS FOR THE SELECTIVE RELEASE OF PERIPLASMIC HUMAN CYSTATIN-C FROM ESCHERICHIA-COLI

Two promising methods, osmotic shock and guanidine treatment, for the selective release of human cystatin C from the periplasmic space of E. coli were developed at the laboratory scale. High release efficiencie s were achievable by both approaches, however, the chemical membrane p ermeabilization proved to be much more selective. Both methods have ex cellent potential for scale up.