PURIFICATION AND CHARACTERIZATION OF A BETA-CYCLODEXTRIN GLUCOSYLTRANSFERASE FROM AN ALKALOPHILIC BACILLUS SP

Authors
YAN TR LIN TT
Citation
Tr. Yan et Tt. Lin, PURIFICATION AND CHARACTERIZATION OF A BETA-CYCLODEXTRIN GLUCOSYLTRANSFERASE FROM AN ALKALOPHILIC BACILLUS SP, Biotechnology techniques, 9(3), 1995, pp. 215-220
Citations number
23
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
3
Year of publication
1995
Pages
215 - 220
Database
ISI
SICI code
0951-208X(1995)9:3<215:PACOAB>2.0.ZU;2-I
Abstract
A beta-cyclodextrin glucosyltransferase was purified from alkalophilic Bacillus sp. No. 562 over 64-fold with a yield of 32%. Its molecular size was estimated to be 170 kDa by gel filtration and 82 kDa by SDS-P AGE, with a pI of 7.2. The enzyme showed optimum activity at 65 degree s C and pH 7.0. It was stable from 0 to 70 degrees C and from pH 7.0 t o 11.0. The enzyme was specifically inhibited by Fe2+ and Fe3+.