A SIMPLE METHOD FOR THE DIRECT EXTRACTION OF PLASMID DNA FROM YEAST

Authors
SOBANSKI MA DICKINSON JR
Citation
Ma. Sobanski et Jr. Dickinson, A SIMPLE METHOD FOR THE DIRECT EXTRACTION OF PLASMID DNA FROM YEAST, Biotechnology techniques, 9(3), 1995, pp. 225-230
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
3
Year of publication
1995
Pages
225 - 230
Database
ISI
SICI code
0951-208X(1995)9:3<225:ASMFTD>2.0.ZU;2-5
Abstract
A rapid and simple method for the small scale isolation of shuttle pla smid DNA from Saccharomyces cerevisiae is described. It uses glass bea ds to break cells and reagents which are also used in bacterial mini-p reps to yield plasmid DNA without chromosomal contamination in suffici ent quantities to enable direct visualisation on agarose gels.