N. Oi et al., ENANTIOMER SEPARATION OF SMALL AMOUNTS OF AMINO-ACIDS AND HYDROXY-ACIDS BY HPLC USING CHIRAL STATIONARY PHASES AND ACHIRAL DERIVATIZING REAGENTS, Bunseki Kagaku, 44(4), 1995, pp. 301-305
The enantiomer separation of small amounts of amino acids and hydroxy
acids by HPLC using chiral stationary phases and achiral derivatizing
reagents was examined. Racemic dabsyl amino acids and pyrene-1-carbony
l hydroxy acids were well resolved on chiral stationary phases [VI] an
d [V], and excellent values of detection limits were obtained. The joi
nt use of chiral stationary phases and achiral derivatizing reagents w
as found to be very effective for the separation and determination of
small amounts of amino acid and hydroxy acid enantiomers by HPLC.