LEWIS-Y ANTIGEN AS DETECTED BY THE MONOCLONAL-ANTIBODY BR96 IS EXPRESSED STRONGLY IN PROSTATIC ADENOCARCINOMA

We used the monoclonal antibody BR96 to determine the expression of th e Lewis Y antigen in benign and malignant prostatic tissues. Strong im muno-staining was detected within the basal cells of benign glands in 29 of 30 specimens examined. In contrast, weak immune-staining of the secretory (luminal) epithelium was detected in only 10 of these same 3 0 specimens. Moderate to strong immuno-staining of luminal cells, howe ver, was observed in prostatic intraepithelial neoplasia in 15 of 17 s pecimens. Immune-staining was detected within the malignant cells in a ll 49 specimens of primary prostatic adenocarcinoma examined. We used a semiquantitative technique to compare the extent of immune-staining among well (combined Gleason score less than 6), moderately (combined Gleason score 6 to 7) and poorly (combined Gleason score more than 7) differentiated tumors as well as metastatic lesions. Poorly differenti ated tumors demonstrated the greatest extent of immune-staining compar ed to moderately and well differentiated adenocarcinoma. Strong immune -staining was also detected within the malignant cells in 7 metastatic (5 nodal lesions and 2 bone marrow biopsies) tumors. The extent of im mune-staining in the metastatic lesions was similar to that observed i n the poorly differentiated primary tumors. In summary, the Lewis Y an tigen, as detected by BR96, is widely expressed within prostatic adeno carcinomas. Furthermore, the poorly differentiated as well as metastat ic lesions frequently demonstrated the highest expression of the Lewis Y antigen.