Ga. Peeters et al., METHOD FOR ISOLATION OF HUMAN VENTRICULAR MYOCYTES FROM SINGLE ENDOCARDIAL AND EPICARDIAL BIOPSIES, American journal of physiology. Heart and circulatory physiology, 37(4), 1995, pp. 1757-1764
The study of adult human ventricular cells has been limited by tissue
availability. In this study we describe techniques for the isolation o
f Ca2+-tolerant adult human ventricular cells from both transvenous en
domyocardial and epicardial biopsies. Ca2+-tolerant cells were obtaine
d from 80% of the biopsies processed. Although the yield of Ca2+-toler
ant myocytes from either type of biopsy was low (1-5%), myocytes with
normal resting potentials and action potentials can be obtained from s
ingle biopsy specimens, providing a source of normal human myocytes fo
r electrophysiological study. Resting potentials (V-rest) were recorde
d in 41 isolated right ventricular endomyocardial cells at 37 degrees
C. Sixteen cells were depolarized (V-rest = -26 +/- 13 mV), and 25 cel
ls had normal resting potentials (V-rest = -84 +/- 6 mV). Action poten
tials were recorded in 16 cells. At a pacing cycle length of 1 s, 4 ce
lls had prolonged action potential duration at 90% (APD(90), 718 +/- 2
6 ms) and 10 cells had normal APD(90) (381 +/- 94 ms) compared with th
ose recorded from intact right ventricular septal trabeculae from expl
anted hearts. Voltage-clamp studies of isolated human ventricular myoc
ytes obtained from these biopsies document the presence of currents pr
eviously reported from cells isolated from explanted hearts.