L. Pirrit et al., CLONING OF THE MAJOR 3-MC INDUCIBLE PHASE-I AND PHASE-II DETOXIFICATION ENZYMES OF PLAICE LIVER, Marine environmental research, 39(1-4), 1995, pp. 15-19
cDNA's coding for cytochrome P4501A1 (CYP1A1), phenol UDP-glucuronosyl
transferase (UDPGT) and glutathione S-transferase (GST-A) were cloned
and sequenced from an expression library prepared from the liver of a
3-methylcholanthrene (3-MC) induced plaice. Plaice CYP1A1 and Phenol U
DPGT display a high degree of structural conservation with homologous
mammalian isoforms and their mRNAs were shown to be highly induced in
liver after 3-MC treatment of fish. Expression of plaice GST-A, which
displays closer homology to GSTs from plants and invertebrates than th
ose of mammals, is repressed after 3-MC treatment.