CLONING OF THE MAJOR 3-MC INDUCIBLE PHASE-I AND PHASE-II DETOXIFICATION ENZYMES OF PLAICE LIVER

Authors
PIRRIT L HEALEY R ASSHETON H LEAVER MJ GEORGE SG
Citation
L. Pirrit et al., CLONING OF THE MAJOR 3-MC INDUCIBLE PHASE-I AND PHASE-II DETOXIFICATION ENZYMES OF PLAICE LIVER, Marine environmental research, 39(1-4), 1995, pp. 15-19
Citations number
11
Categorie Soggetti
Marine & Freshwater Biology","Environmental Sciences",Toxicology
Journal title
Marine environmental research → ACNP
ISSN journal
01411136
Volume
39
Issue
1-4
Year of publication
1995
Pages
15 - 19
Database
ISI
SICI code
0141-1136(1995)39:1-4<15:COTM3I>2.0.ZU;2-P
Abstract
cDNA's coding for cytochrome P4501A1 (CYP1A1), phenol UDP-glucuronosyl transferase (UDPGT) and glutathione S-transferase (GST-A) were cloned and sequenced from an expression library prepared from the liver of a 3-methylcholanthrene (3-MC) induced plaice. Plaice CYP1A1 and Phenol U DPGT display a high degree of structural conservation with homologous mammalian isoforms and their mRNAs were shown to be highly induced in liver after 3-MC treatment of fish. Expression of plaice GST-A, which displays closer homology to GSTs from plants and invertebrates than th ose of mammals, is repressed after 3-MC treatment.