ALKOXYQUINOLINE O-DEALKYLATION - A NEW FLUOROMETRIC ASSAY FOR HYDROCARBON-INDUCED CYTOCHROME-P450 IN FISH

Authors
BURKE MD BROWN D MAYER RT HOULIHAN DFJ
Citation
Md. Burke et al., ALKOXYQUINOLINE O-DEALKYLATION - A NEW FLUOROMETRIC ASSAY FOR HYDROCARBON-INDUCED CYTOCHROME-P450 IN FISH, Marine environmental research, 39(1-4), 1995, pp. 57-60
Citations number
9
Categorie Soggetti
Marine & Freshwater Biology","Environmental Sciences",Toxicology
Journal title
Marine environmental research → ACNP
ISSN journal
01411136
Volume
39
Issue
1-4
Year of publication
1995
Pages
57 - 60
Database
ISI
SICI code
0141-1136(1995)39:1-4<57:AO-ANF>2.0.ZU;2-V
Abstract
The O-dealkylation of a new series of substrates, the alkoxyquinolines , provides a new easy fluorimetric assay for measuring the induction o f cytochrome P450 by polycyclic aromatic hydrocarbons and drilling mud oil in fish. Expression of the results as a ratio between the inducib le butyloxyquinoline O-dealkylation (BuQOD) activity and the non-induc ible benzyloxyquinoline O-dealkylation (Bel)OD) activity (i.e. the 'Bu QOD:- BeQOD quotient') maximises the degree of induction measured and minimises the degree of inter-animal variation.