SILVER ION-SELECTIVE OPTODES BASED ON NOVEL THIA ETHER COMPOUNDS

Flow-through type fiber-optic silver ion selective optodes based on no vel cyclic and noncyclic thia crown ether compounds and a Lipophilic a nionic indicator dye have been developed. Pellicular octadecylsilica b eads were coated with a lipophilic organic liquid containing the thia ether compound as the silver ion selective ionophore and LAD-3 as the color changeable indicator dye, The optode system was constructed by p acking these color changeable ion sensing beads ina now-through optica l cell (cell volume, 7 mu L) having a quartz window attached directly to the distal end of a bifurcated optical fiber. The optode based on a lipophilic thia crown ether that has two sulfur atoms (tetradecyl-14- dithiacrown-4) exhibited the most sensitive response to silver ion of all the optodes investigated. This optode could detect silver ion in c oncentrations ranging from 10(-6) to 10(-2) M at pH 7.0 with 0.05 M Tr is-H2SO4 buffer as the flowing solution by measuring the absorbance ch ange in the sensing beads at 516 mm. This optode showed a higher selec tive response to Ag+ without any interference from other transition me tal ions, except for Hg2+, up to 10(-3) M, as well as alkali metal and alkaline earth metal ions up to 1 M. Optodes employing highly lipophi lic ionophores showed good reproducible response to Ag+ for at least 2 weeks.