PHENYLGUANINE FOUND IN URINE AFTER BENZENE EXPOSURE

Comparative investigations with synthetic N-7-phenylguanine were carri ed out to clarify whether this compound is eliminated via the urine of rats as a benzene-derived nucleic acid adduct. As sensitive methods f or detecting trace amounts of the compound, gas chromatography-mass sp ectroscopy, high performance liquid chromatography, and two immunoassa ys (enzyme-linked immunosorbent assay and fluoroimmunoassay) with appr opriate monoclonal antibodies were used. The results indicate the excr etion of several benzene-related guanine adducts slightly different fr om N-7-phenylguanine that may possibly be bydroxylated. These adducts differ also from O-6-, N-2- and C8-phenylguanine, respectively.