J. Winieckakrusnell et E. Linder, DETECTION OF GIARDIA-LAMBLIA CYSTS IN STOOL SAMPLES BY IMMUNOFLUORESCENCE USING MONOCLONAL-ANTIBODY, European journal of clinical microbiology & infectious diseases, 14(3), 1995, pp. 218-222
The diagnostic potential of indirect immunofluorescence to detect Giar
dia cysts in stool samples using a cyst-specific anti-Giardia lamblia
monoclonal antibody was evaluated in comparison to conventional light
microscopy. One hundred fifty specimens from clinically suspected Giar
dia infections and 50 control samples from microscopically proved Giar
dia infections were tested, Giardia cysts were found in 15 of 150 (10
%) samples tested by light microscopy, whereas immunofluorescence micr
oscopy detected 35 of 150 (23 %) positive samples. Forty-six of the 50
reference samples previously shown to contain Giardia cysts were posi
tive. Apparently, the four discrepant samples contained very low numbe
rs of parasites, as none could be detected by conventional microscopy.
The results show that Giardia lamblia cysts are detected significantl
y more frequently using the antibody marker. The doubled number of pos
itive stool specimens and detection of as little as four cysts per sam
ple suggest that microscopical examination of samples can be improved
by immunofluorescent staining of Giardia lamblia cysts.