PRESENCE OF HELICOBACTER-PYLORI IN THE ORAL CAVITY, ESOPHAGUS, STOMACH AND FECES OF PATIENTS WITH GASTRITIS

Citation
F. Namavar et al., PRESENCE OF HELICOBACTER-PYLORI IN THE ORAL CAVITY, ESOPHAGUS, STOMACH AND FECES OF PATIENTS WITH GASTRITIS, European journal of clinical microbiology & infectious diseases, 14(3), 1995, pp. 234-237
Citations number
13
Categorie Soggetti
Immunology,Microbiology
ISSN journal
09349723
Volume
14
Issue
3
Year of publication
1995
Pages
234 - 237
Database
ISI
SICI code
0934-9723(1995)14:3<234:POHITO>2.0.ZU;2-4
Abstract
The presence of Helicobacter pylori in the oral cavity (6 sites), oeso phagus, stomach and bowel of 20 dyspeptic patients was investigated, S amples were cultured on three selective media and analyzed by 16S rDNA polymerase chain reaction (PCR) and southern hybridization. Helicobac ter pylori DNA was detected by PCR from oral-cavity samples of three ( 20 %) and from faeces samples of only one (7 %) of the patients whose stomach biopsies were positive for Helicobacter pylori. When culture w as used, the microorganism's rate of recovery from the oral cavity and faeces was 13 % and 7 %, respectively. One patient had a Helicobacter pylori-like organism in samples collected from the tongue and palate. Both strains were urease, catalase and oxidase positive and grew micr oaerophilically but were negative on PCR analysis. This demonstrates t he possibility of false identification of Helicobacter pylori by use o f routine enzyme reactions. Interestingly, specimens collected from th e cheeks of three patients were positive for Helicobacter pylori by PC R analysis. This is the first instance of detection of this microorgan ism in the cheek.