Hd. Viken et al., THE CYTOTOXIC HLA-DQ3 REACTIVE HUMAN HYBRIDOMA ANTIBODY-4166 THAT MAYDISTINGUISH DQ7+8 FROM DQ9, Human immunology, 42(4), 1995, pp. 281-288
The human cytotoxic hybridoma antibody 4166 (IgM[x]) was generated by
fusing an in vitro EBV-transformed B-LCL from a multiparous woman with
the mouse-human heteromyeloma fusion partner CB-F7. In microcytotoxic
ity and IIF tests with B-LCLs as target cells, the mAb 4166 was specif
ic for DQ3 (= DQ7+8+9). However, when used for DQ typing of class-II-p
ositive PBMCs, 4166 could be rendered functionally specific for DQ7+8
and did not react with DQ9(+) PBMCs. Binding of mAb 4166 to DQ8-positi
ve cells was efficiently blocked by several allotype-specific mAbs rec
ognizing DQ8. Other HLA class-II-specific mAbs were unable to inhibit.
With the use of mAb 4166, it is possible to discriminate DQ7+8 from D
Q9 in serologic DQ typing.