HOMOLOGS OF GENES AND ANONYMOUS LOCI ON HUMAN-CHROMOSOME 13 MAP TO MOUSE CHROMOSOME-8 AND CHROMOSOME-14

To enhance the comparative map for human Chromosome (Chr) 13, we ident ified clones for human genes and anonymous loci that cross-hybridized with their mouse homologs and then used linkage crosses for mapping. O f the clones for four genes and twelve anonymous loci tested, cross-hy bridization was found for six, COL4A1, COL4A2, D13S26, D13S35, F10, an d PCCA. Strong evidence for homology was found for COL4A1, COL4A2, D13 S26, D13S35, and F10, but only circumstantial homology evidence was ob tained for PCCA. To genetically map these mouse homologs (Cf10, Col4a1 , Col4a2, D14H13S26, D8H13S35, and Pcca-rs), we used interspecific and intersubspecific mapping panels. D14H13S26 and Pcca-rs were located o n the distal portion of mouse Chr 14 extending by similar to 30 cM the conserved linkage between human Chr 13 and mouse Chr 14, assuming tha t Pcca-rs is the mouse homolog of PCCA. By contrast, Cf10, Col4a1, Col 4a2, and D8H13S35 mapped near the centromere of mouse Chr 8, defining a new conserved linkage. Finally, we identified either a closely linke d sequence related to Col4a2, or a recombination hot-spot between Col4 a1 and Col4a2 that has been conserved in humans and mice.