A GENETIC-MAP OF MICROSATELLITE MARKERS ON RAT CHROMOSOME-7

Nine microsatellite loci were mapped to rat Chromosome (Chr) 7 by gene tic linkage and somatic cell hybrid analysis. These loci include the g ene encoding a member of the IID subfamily of cytochrome P450 (Cyp2d), a gene with repetitive sequences expressed during myotube formation ( D7Arble), four anonymous loci, D7Arb81, D7Arb208, D7Arb569, D7Arb609a, and three DNA loci defined by MapPair(TM) markers R245, R513, and R10 71. The nine loci were all identified by PCR-based microsatellite poly morphism analysis and were characterized in 40 F-2 intercross progeny of Fischer (F344/N) and Lewis (LEW/N) rats for segregation analysis. T hese markers formed a single linkage group spanning 76.8 cM with the f ollowing order and distances: D7Arb569-11.4 cM-D7Arb81-9.7 cM-R513-2.6 cM-Cyp2d-0.0 cM-R245-1.3 cM-D7Arble-10.4 cM-R1071-15.9 cM-D7Arb609a-1 5.4 cM-D7Arb208. Physical mapping of Cyp2d by somatic cell hybrid anal ysis allowed us to assign this linkage group to rat Chr 7. For each ma rker, two to six alleles were detected in a panel of 16 inbred rat str ains (ACI/N, BN/SsN, BUF/N, DA/Bkl, F344/N, LER/N, LEW/N, LOU/MN, MNR/ N, MR/N, SHR/N, SR/Jr, SS/Jr, WBB1/N, WBB2/N, WKY/N).