ELECTROPHORETIC MOBILITY OF DNA IN GELS .3. EXPERIMENTAL-STUDY ON BAND INVERSION

A series of experiments has been undertaken to study the phenomenon of band inversion that can occur during the separation of linear double- stranded DNA in agarose gels under constant electric field. We found t hat there can be a considerable band inversion when the DNA fragments ape moving as a single species. When separating mixtures of fragments, as it is usually done in routine experiments, the band inversion effe ct is strongly reduced. Our data support the assumption that DNA-DNA i nteractions can play an important role in electrophoretic separations. (C) 1995 John Wiley & Sons, Inc.