PRODUCTION OF A NEW MONOCLONAL-ANTIBODY SPECIFIC TO HUMAN DES-GAMMA-CARBOXYPROTHROMBIN IN THE PRESENCE OF CALCIUM-IONS - APPLICATION TO THEDEVELOPMENT OF A SENSITIVE ELISA-TEST

Authors
BELLE M BREBANT R GUINET R LECLERCQ M
Citation
M. Belle et al., PRODUCTION OF A NEW MONOCLONAL-ANTIBODY SPECIFIC TO HUMAN DES-GAMMA-CARBOXYPROTHROMBIN IN THE PRESENCE OF CALCIUM-IONS - APPLICATION TO THEDEVELOPMENT OF A SENSITIVE ELISA-TEST, Journal of immunoassay, 16(2), 1995, pp. 213-229
Citations number
16
Categorie Soggetti
Immunology
Journal title
Journal of immunoassay → ACNP
ISSN journal
01971522
Volume
16
Issue
2
Year of publication
1995
Pages
213 - 229
Database
ISI
SICI code
0197-1522(1995)16:2<213:POANMS>2.0.ZU;2-X
Abstract
In order to explore pathologies possibly associated with vitamin K def iciency, several monoclonal antibodies (mAbs) were produced against hu man Desgamma-Carboxy-Prothrombin (DCP). One of these mAbs, designated C4B6, detected DCP forms in the presence of Calcium ions, confirmed by comparison with the patterns of two electrophoretic techniques : Affi no-Immuno-Electrophoresis (CAIE) and Polyacrylamide Gel Electrophoresi s followed by Electro-blotting (PAGE-Blot). An Enzyme-Linked-Imunosorb ent Assay (ELISA) using mAb C4B6 has been developed, optimized and sta ndardized. It has proven to be specific for DCP forms and has a minimu m sensivity of 0.156 A.U/ml.