C. Overmeyer et Hj. Rehm, BIODEGRADATION OF 2-CHLOROETHANOL BY FREELY SUSPENDED AND ADSORBED IMMOBILIZED PSEUDOMONAS-PUTIDA US2 IN SOIL, Applied microbiology and biotechnology, 43(1), 1995, pp. 143-149
The degradation of 2-chloroethanol by Pseudomonas putida US2 was inves
tigated in batch, repeated batch and continuous cultures especially in
a packed-bed fermenter with sand. The degradation of 2-chloroethanol
was connected with a release of protons, which led to a decrease of th
e pH in the medium. Higher initial concentration than 25 mM 2-chloroet
hanol were not degraded completely because they entailed a decrease of
the pH to 5.0, which inhibited further growth and degradation. P. put
ida US2 showed a typical repression of catabolites and diauxic growth
with succinate as cosubstrate. The addition of succinate as a second s
ubstrate caused a decrease in degradation of 2-chloroethanol. Activate
d sludge added to adsorbed cultures in a continuous fermentation did n
ot lead to a decrease in metabolic activity. After 2 weeks of continuo
us cultivation the specialized strain could be retained.