Pd. Burr et al., DETECTION OF CANINE HERPESVIRUS-1 IN A WIDE-RANGE OF TISSUES USING THE POLYMERASE CHAIN-REACTION, Veterinary microbiology, 53(3-4), 1996, pp. 227-237
Canine herpesvirus 1 (CHV-1), a member of the alphaherpesvirus sub-fam
ily, is known to cause fatal infections in litters of puppies and may
also be involved in infertility, abortion, and stillbirths in adult do
gs. The purpose of this study was to determine the presence of CHV-1 D
NA using the polymerase chain reaction (PCR) in twelve key sites that
have been associated with latency for other herpesviruses. A 605 base
pair portion of the viral glycoprotein B (gB) gene was amplified using
degenerate primers, cloned, and sequenced. Conventional 20mer primers
were designed using this sequence information to amplify a 120 bp fra
gment of gB situated between the original degenerate primers. The spec
ificity of amplification was confirmed by Southern Blot hybridisation
using an internal oligonucleotide probe. DNA was extracted from tissue
samples taken from twelve dogs at post mortem and from twenty-four bl
ood samples. Nine out of twelve dogs showed evidence of infection with
CHV-1; the tissues most commonly affected were lumbo-sacral ganglia (
5/12 dogs), tonsil (5/12), parotid salivary gland (4/9), and liver (4/
9). No positive results were detected within the twenty-four blood sam
ples. These results indicate that exposure to CHV-1 may be much more c
ommon than previously suggested.