BIOSENSING EFFECTS IN FUNCTIONALIZED ELECTROCONDUCTING CONJUGATED POLYMER LAYERS - ADDRESSABLE DNA MATRIX FOR THE DETECTION OF GENE-MUTATIONS

A new methodology for the preparation of addressed DNA matrices is des cribed. The process is based on a one-step electrodeposition of a poly pyrrole film functionalized by a covalently linked oligonucleotide. Ev en in the bulk of the film the single-stranded DNA fragment can be hyb ridized or denatured. A support bearing four oligonucleotide spots was obtained by successive copolymerization on a four electrode matrix. T his device allows detection of three ras mutations on a synthetic DNA fragment without cross contamination.