Inorganic and organic soil P (P-i, P-o) fractions were followed monthl
y for 15 months in a 100-year-old, fertilization and crop-rotation exp
eriment with the Rubaek-Sibbesen, macroporous resin method, the Olsen
method, and the Hedley fractionation method. Resin P, and Olsen P had
similar levels and variation patterns. They increased in spring after
fertilization, decreased during summer and autumn, and increased again
in winter after repeated slurry applications. Resin P-o decreased in
spring and peaked in summer. The variation in time of the Hedley P-i a
nd P-o fractions was relatively smaller and was neither related to sea
son nor to fertilization. Un-manured soil contained much less total P
than NPK and slurry-treated soils, but the differences in total P-i we
re greater than those in total P-o. Neither total P-i nor total P-o co
ncentrations differed between NPK and slurry treatments, indicating th
at P-o in animal manure is quickly mineralized. All P-i and P-o fracti
ons were smaller in unmanured than in fertilized treatments. These dif
ferences were relatively largest for resin P-i and resin P-o, i.e., th
e most labile fractions, and decreased for the medium and less labile
P-i and P-o fractions. The reactions by resin P-i, Olsen P-o and resin
P-o to seasons and treatments indicate that these fractions are estim
ates of the most labile pools of P-i and P-o in soil, which make them
relevant for shortterm studies. The medium and less labile P-i and P-o
fractions of the Hedley fractionation method seem more relevant for l
ong-term studies.