Jj. Lynch et al., SODIUM-CHANNEL BLOCKERS REDUCE OXYGEN-GLUCOSE DEPRIVATION-INDUCED CORTICAL NEURONAL INJURY WHEN COMBINED WITH GLUTAMATE-RECEPTOR ANTAGONISTS, The Journal of pharmacology and experimental therapeutics, 273(1), 1995, pp. 554-560
Blockers of voltage-gated Na+ channels can protect central neuronal ax
ons from hypoxic injury in vitro but have shown limited neuroprotectiv
e effects on neurons, where substantial injury is mediated by glutamat
e receptors. We explored the ability of several voltage-gated Na+ chan
nel blockers to protect murine cultured cortical neurons from injury i
nduced by oxygen-glucose deprivation. Whole-cell recordings from neuro
ns revealed two types of Na+ currents activated by membrane depolariza
tion: one rapidly inactivating and the other noninactivating. Both cur
rents were blocked by tetrodotoxin (TTX) and 5,5-diphenylhydantoin (ph
enytoin). Fluorescent imaging using the Na+-selective dye SBFI confirm
ed that TTX attenuated the increase in intracellular free Na+ induced
by oxygen-glucose deprivation. Addition of TTX (1 mu M) but not phenyt
oin (10-100 mu M) produced a small and variable reduction in neuronal
death subsequent to oxygen-glucose deprivation for 40 to 50 min, Block
ade of glutamate neurotoxicity by combined addition of MK-801, 7-chlor
okynurenate and 6-cyano-7-nitroquinoxaline-2,3-dione markedly reduced
injury such that prolonged deprivation times (75-100 min) were needed
to induce widespread neuronal death. In this setting of glutamate rece
ptor blockade, addition of mt, phenytoin or one of several other Na+ c
hannel blockers-lidocaine (100 mu M), QX-314 (1 mM), quinidine (100 mu
M) or lorcainide (10 or 100 mu M)-all further reduced neuronal death.
Present results raise the possibility that Na+ channel blockers may b
e useful in protecting gray matter from hypoxic-ischemic injury, espec
ially when combined with antiexcitotoxic approaches.