AN AUTOMATED SENSITIVE ELISA TO MEASURE IGE PRODUCED BY CULTURED HUMAN-LYMPHOCYTES

An automated sandwich enzyme-linked immunosorbent assay (ELISA) to mea sure human immunoglobulin E (IgE) in the concentration range of 0.2 to 100 ng/mL was developed. The ELISA uses a rabbit antihuman IgE for th e capture antibody, a biotinylated goat antihuman IgE for the defectio n antibody, and alkaline phosphatase conjugated to streptavidin as the detection system. Assay precision was improved over manual methods an d had a coefficient of variation (CV) of 3-8%. Accuracy was confirmed by measurement of standards calibrated against World Health Organizati on (WHO) standards. Cross-reactivity against other human immunoglobuli ns was less than 0.001%. This ELISA successfully measured IgE concentr ations of culture supernatants from human lymphocytes.