HERITABLE FORMATION OF NEUROECTODERMAL TUMOR IN TRANSGENIC MICE CARRYING THE COMBINED E1 REGION GENE OF ADENOVIRUS-TYPE-12 WITH THE DEREGULATED HUMAN RENIN PROMOTER
F. Sugiyama et al., HERITABLE FORMATION OF NEUROECTODERMAL TUMOR IN TRANSGENIC MICE CARRYING THE COMBINED E1 REGION GENE OF ADENOVIRUS-TYPE-12 WITH THE DEREGULATED HUMAN RENIN PROMOTER, Journal of cellular biochemistry, 57(4), 1995, pp. 691-700
Adenovirus early 1 (E1) region gene products, including E1A and E1B, a
re required for transcriptional regulation of viral and cellular promo
ters in infected and transfected culture cells and for transformation
of primary rodent cells. Here, we established a line of transgenic mic
e carrying the E1 region gene of human adenovirus type 12 under the co
ntrol of the human renin promoter, in which a neuroectodermal tumor de
rived from retroperitoneal, olfactory, and/or pelvic regions was herit
ably developed with varying degrees of incidence and the phenotype was
successfully passed through six generations. The transgenes were loca
ted in the region E2-E3 bands of chromosome 7 with which no genetic li
nkage to neuroectodermal tumors was previously demonstrated, and expre
ssed only in the tumors but not in another tissue examined. Notably, i
n addition to the expression of a neural marker gene N-CAM, the three
nuclear oncogenes, c-, L-, and N-myc, were coexpressed in the tumors.
These results suggest that E1A and E1B are cooperatively involved in t
he heritable formation of neuroectodermal tumors associated with co-ex
pression of the three sets of myc family genes. (C) 1995 Wile-Liss, In
c.