AFFINITY-PURIFICATION AND IDENTIFICATION OF GRPE HOMOLOGS FROM MAMMALIAN MITOCHONDRIA

Authors
NAYLOR DJ RYAN MT CONDRON R HOOGENRAAD NJ HOJ PB
Citation
Dj. Naylor et al., AFFINITY-PURIFICATION AND IDENTIFICATION OF GRPE HOMOLOGS FROM MAMMALIAN MITOCHONDRIA, Biochimica et biophysica acta. Protein structure and molecular enzymology, 1248(1), 1995, pp. 75-79
Citations number
26
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Protein structure and molecular enzymology → ACNP
ISSN journal
01674838
Volume
1248
Issue
1
Year of publication
1995
Pages
75 - 79
Database
ISI
SICI code
0167-4838(1995)1248:1<75:AAIOGH>2.0.ZU;2-A
Abstract
We used affinity chromatography on DnaK columns to identify a mitochon drial GrpE homologue from bovine, porcine and rat liver mitochondria. The 24 kDa GrpE homologue bound specifically to the DnaK column and wa s not eluted with 1 M KCl but readily with 5 mM ATP. Sequence analysis of the bovine homologue (85 residues) revealed 42% positional identit y to mitochondrial GrpEp from S. cerevisiae and about 30% identity to the bacterial counterparts. Thus, GrpE homologues from higher and lowe r eukaryotes are highly conserved.