PCR AMPLICON RESTRICTION-ENDONUCLEASE ANALYSIS OF THE CHROMOSOMAL DHPS GENE OF NEISSERIA-MENINGITIDIS - A METHOD, FOR STUDYING SPREAD OF THE DISEASE-CAUSING STRAIN IN CONTACTS OF PATIENTS WITH MENINGOCOCCAL DISEASE
Be. Kristiansen et al., PCR AMPLICON RESTRICTION-ENDONUCLEASE ANALYSIS OF THE CHROMOSOMAL DHPS GENE OF NEISSERIA-MENINGITIDIS - A METHOD, FOR STUDYING SPREAD OF THE DISEASE-CAUSING STRAIN IN CONTACTS OF PATIENTS WITH MENINGOCOCCAL DISEASE, Journal of clinical microbiology, 33(5), 1995, pp. 1174-1179
We tested two sets of primers derived from the dhps gene of Neisseria
meningitidis for the amplification of meningococcal DNA by PCR, Both t
he NM1-NM6 primers and the NM3-NM6 primers amplified dhps DNA from all
of the meningococci included in the study, resulting, in most cases,
in amplicons of 0.70 and 0.23 kb, respectively. Also, dhps DNAs of N.
gonorrhoeae and some commensals were amplified but Haemophilus influen
zae, Streptococcus pneumoniae, and Escherichia coli DNAs were not. By
PCR amplicon restriction endonuclease analysis (AREA) of the larger am
plicon, we could differentiate between individual strains of N. mening
itidis, Following two cases of meningococcal disease, we used PCR AREA
to identify healthy contacts carrying the disease-causing strain, We
conclude that PCR AREA is a useful method for meningococcal strain dif
ferentiation and that it has potential as a method for studying the sp
read of a disease-causing strain in an affected population. The method
is quicker and easier to perform and interpret than chromosomal DNA f
ingerprinting.