Me. Lozano et al., RAPID DIAGNOSIS OF ARGENTINE HEMORRHAGIC-FEVER BY REVERSE-TRANSCRIPTASE PCR-BASED ASSAY, Journal of clinical microbiology, 33(5), 1995, pp. 1327-1332
Argentine hemorrhagic fever (AHF) is an endemo-epidemic disease caused
by Junin virus. This report demonstrates that a reverse transcriptase
(RT) PCR-based assay developed in our laboratory to detect Junin viru
s in whole blood samples is sensitive and specific. The experiments we
re conducted in a double-blinded manner using 94 clinical samples coll
ected in the area in which AHF is endemic. The RT-PCR-based assay was
compared with traditional methodologies, including enzyme-linked immun
osorbent assay, plaque neutralization tests, and occasionally viral is
olation, The calculated parameters for RT-PCR diagnosis, with seroconv
ersion as the ''gold standard,'' were 98% sensitivity and 76% specific
ity. It is noteworthy that 94% of the patients with putative false-pos
itive results (RT-PCR positive and no seroconversion detected) exhibit
ed febrile syndromes of undefined etiology. These results could be int
erpreted to mean that most of those patients with febrile syndromes we
re actually infected with Junin virus but did not develop a detectable
immune response. Furthermore, 8 laboratory-fabricated samples and 25
blood samples of patients outside the area in which AHF is endemic tes
ted in a similar way were disclosed correctly (100% match). The RT-PCR
assay is the only laboratory test available currently for the early a
nd rapid diagnosis of AHF. It is sensitive enough to detect the low vi
remia found during the period in which immune plasma therapy tan be us
ed effectively, reducing mortality rates from 30% to less than 1%.