Js. Boyle et al., DETECTION OF THE TURKEY PATHOGENS MYCOPLASMA-MELEAGRIDIS AND M-IOWAE BY AMPLIFICATION OF GENES-CODING FOR RIBOSOMAL-RNA, Journal of clinical microbiology, 33(5), 1995, pp. 1335-1338
PCR-based diagnostic tests using oligonucleotides specific to 16S rRNA
were designed for the specific detection of the turkey pathogens Myco
plasma meleagridis and M. iowae. This method of detection was shown to
be rapid, species specific, and unaffected by strain variation or the
presence of other organisms. Detection of M. meleagridis in clinical
samples by PCR was achieved and later confirmed by culture and growth
inhibition. Definitive identification by culture and growth inhibition
required up to 3 weeks, whereas positive results from PCR testing wer
e obtained within a day and negative samples were confirmed within 4 d
ays.