DIAGNOSTIC ASSAY FOR HELICOBACTER-HEPATICUS BASED ON NUCLEOTIDE-SEQUENCE OF ITS 16S RIBOSOMAL-RNA GENE

Authors
BATTLES JK WILLIAMSON JC PIKE KM GORELICK PL WARD JM GONDA MA
Citation
Jk. Battles et al., DIAGNOSTIC ASSAY FOR HELICOBACTER-HEPATICUS BASED ON NUCLEOTIDE-SEQUENCE OF ITS 16S RIBOSOMAL-RNA GENE, Journal of clinical microbiology, 33(5), 1995, pp. 1344-1347
Citations number
44
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
33
Issue
5
Year of publication
1995
Pages
1344 - 1347
Database
ISI
SICI code
0095-1137(1995)33:5<1344:DAFHBO>2.0.ZU;2-E
Abstract
Conserved primers were used to PCR amplify 95% of the Helicobacter hep aticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to highly dive rged regions of the 16S rRNA gene and an oligonucleotide probe for the development of a PCR-liquid hybridization assay. This assay was shown to be both sensitive and specific for H. hepaticus 16S rRNA gene sequ ences.